AAVs in Focus: Innovative Approaches to Guide Cutting-Edge Gene Therapy
This Article Collection delves into the latest research and applications of adeno-associated viruses (AAVs), highlighting advancements in production, characterization, and purification processes. By examining these studies, we aim to provide a comprehensive overview of the current status and future prospects of AAV technology in therapeutic applications.
What you will learn about:
- Stability & Delivery: Enhance AAV stability and delivery with innovative shielding techniques.
- Transcriptomics: Discover molecular signatures for rAAV production.
- mRNA-Sequencing: In-depth analysis of cell lines during gene therapy processes.
- Purification: Simplified anion exchange techniques for separating full and empty AAV capsids.
More Information
Adeno-associated viruses (AAVs) have emerged as pivotal tools in gene therapy due to their ability to deliver genetic material safely and efficiently. This article collection begins with a comprehensive review of the current research status and applications of AAVs. It includes studies on the reversible shielding and immobilization of liposomes and viral vectors by tailored antibody-ligand interactions, transcriptomic features revealing molecular signatures associated with recombinant AAV production, and mRNA-sequencing-based characterization of HEK-293 cell lines during rAAV production. Additionally, it introduces a novel anion exchange flow-through polishing approach for the separation of full from empty AAV capsids, promising to enhance the purity and efficacy of AAV preparations.
Articles contained in the collection:
- Xia, K. et al. (2025). Research Status and Applications of Adeno-Associated Virus. ChemBioChem. https://doi.org/10.1002/cbic.202400856.
- Thomas, O.S. et al. (2022). Reversible Shielding and Immobilization of Liposomes and Viral Vectors by Tailored Antibody-Ligand Interactions. Small. https://doi.org/10.1002/smll.202105157.
- Wang, Y. et al. (2023). Transcriptomic features reveal molecular signatures associated with recombinant adeno-associated virus production in HEK293 cells. Biotechnology Progress. https://doi.org/10.1002/btpr.3346.
- Pistek, M. et al. (2023). Comprehensive mRNA-sequencing-based characterization of three HEK-293 cell lines during an rAAV production process for gene therapy applications. Biotechnology Journal. https://doi.org/10.1002/biot.202200513.
- Meierrieks, F. et al. (2024). A Novel and Simplified Anion Exchange Flow-Through Polishing Approach for the Separation of Full From Empty Adeno-Associated Virus Capsids. Biotechnology Journal. https://doi.org/10.1002/biot.202400430.
