U.S. chemicals group DuPont said it was confident its increased $6.4 billion offer for Denmark's Danisco would succeed, after a hedge fund stoked uncertainty over the takeover.

DuPonte affirmed on Tuesday that its revised bid of 700 Danish crowns ($134.1) per share for Danisco was its "best and final" offer.
The statement followed news last week that U.S. hedge funds group Elliott International had raised its stake in Danisco above 10%, making it the largest shareholder. Elliott rejected DuPont's initial 665 crown offer as too low, but has not made clear what it thinks of the higher bid.

DuPont raised its bid for Danisco on April 29 and lowered the level of acceptance that it requires of Danisco shareholders to 80% from 90% to persuade reluctant stockholders to take the offer.

"All major Danish institutional investors have expressed support for our revised offer and we remain confident that the tender will be completed successfully on May 13," DuPont Chief Executive Ellen Kullman said in a statement on Tuesday.

"As the deadline approaches, we want to ensure that there is no confusion in the market regarding our offer or our intentions," Kullman said. "These terms represent our best and final offer."

"To be clear, we will not raise our price or further amend or extend our offer," she added.

Kullman noted that the Danish Financial Supervisory Authority has made it clear that Danish regulations prevent DuPont from making any further changes to the terms of the deal.

She said that in the "unlikely event" that the current offer did not succeed, DuPont would move on to "aggressively pursue other strategic opportunities that we've been exploring."

DuPont said in the statement that tendering shares is the only way for Danisco shareholders to receive the full value and certainty of the 700-crowns cash offer. The offer will expire on Friday at 11 p.m. local time, DuPont said.